tailieunhanh - Báo cáo y học: "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors? | Open Access Research Interrupted coding sequences in Mycobacterium smegmatis authentic mutations or sequencing errors Caroline Deshayes Emmanuel Perrodou Sebastien Gallien Daniel Euphrasie Christine Schaeffer Alain Van-Dorsselaer Olivier Poch Odile Lecompte and Jean-Marc Reyrat Addresses Université Paris Descartes Faculte de Médecine René Descartes Paris Cedex 15 F-75730 France. Inserm U570 Unite de Pathogénie des Infections Systémiques-Groupe AVENIR Paris Cedex 15 F-75730 France. Laboratoire de Biologie et Génomique Structurales IGBMC CNRS INSERM ULP BP 163 67404 Illkirch Cedex France. Laboratoire de Spectrométrie de Masse Bio-Organique UMR7178 ECPM rue Becquerel Strasbourg F-67087 cedex 2 France. Correspondence Jean-Marc Reyrat. Email jmreyrat@ Published 12 February 2007 Genome Biology 2007 8 R20 doi 186 gb-2007-8-2-r20 The electronic version of this article is the complete one and can be found online at http 2007 8 2 R20 Received 7 September 2006 Revised 20 November 2006 Accepted 12 February 2007 2007 Deshayes et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In silico analysis has shown that all bacterial genomes contain a low percentage of ORFs with undetected frameshifts and in-frame stop codons. These interrupted coding sequences ICDSs may really be present in the organism or may result from misannotation based on sequencing errors. The reality or otherwise of these sequences has major implications for all subsequent functional characterization steps including module prediction comparative genomics and high-throughput proteomic projects. Results We show here using Mycobacterium smegmatis as a model species that a significant proportion of these ICDSs result .