tailieunhanh - Báo cáo y học: "Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes. | Open Access Research Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes Yves Gibon Bjoern Usadel Oliver E Blaesing Beate Kamlage Melanie Hoehne Richard Trethewey and Mark Stitt Addresses Max Planck Institute of Molecular Plant Physiology Science Park Golm Am Muehlenberg D-14476 Potsdam-Golm Germany. tmetanomics GmbH Tegeler Weg 10589 Berlin Germany. Correspondence Yves Gibon. Email gibon@ Published 17 August 2006 Genome Biology 2006 7 R76 doi l86 gb-2006-7-8-r76 The electronic version of this article is the complete one and can be found online at http 2006 7 8 R76 Received 11 May 2006 Revised 22 June 2006 Accepted 17 August 2006 2006 Gibon et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genome-wide transcript profiling and analyses of enzyme activities from central carbon and nitrogen metabolism show that transcript levels undergo marked and rapid changes during diurnal cycles and after transfer to darkness whereas changes in activities are smaller and delayed. In the starchless pgm mutant where sugars are depleted every night there are accentuated diurnal changes in transcript levels. Enzyme activities in this mutant do not show larger diurnal changes instead they shift towards the levels found in the wild type after several days of darkness. This indicates that enzyme activities change slowly integrating the changes in transcript levels over several diurnal cycles. Results To generalize this conclusion 137 metabolites were profiled using gas and liquid chromatography coupled to mass spectroscopy. The amplitudes of the diurnal changes in metabolite levels in pgm were with the exception of .