tailieunhanh - Báo cáo y học: "An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta. | Open Access Method An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta John WangH Stephanie JemielityH Paolo Uva Yannick Wurm Johannes Graff and Laurent Keller Addresses Department of Ecology and Evolution University of Lausanne 1015 Lausanne Switzerland. fIstituto di Ricerche di Biologia Molecolare Merck Research Laboratories 00040 Pomezia Rome Italy. Brain Research Institute University of Zurich Swiss Federal Institute of Technology 8057 Zurich Switzerland. H These authors contributed equally to this work. Correspondence John Wang. Email Published 15 January 2007 Genome Biology 2007 8 R9 doi gb-2007-8-1-r9 The electronic version of this article is the complete one and can be found online at http 2007 8 1 R9 Received 29 June 2006 Revised 17 November 2006 Accepted 15 January 2007 2007 Wang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Ants display a range of fascinating behaviors a remarkable level of intra-species phenotypic plasticity and many other interesting characteristics. Here we present a new tool to study the molecular mechanisms underlying these traits a tentatively annotated expressed sequence tag EST resource for the fire ant Solenopsis invicta. From a normalized cDNA library we obtained 21 715 ESTs which represent 11 864 putatively different transcripts with very diverse molecular functions. All ESTs were used to construct a cDNA microarray. Background Ants are important model species for sociobiology and behavioral ecology 1 . Life in an ant colony is marked by cooperation but it also harbors conflicts. Both aspects have been studied extensively to understand the prerequisites for social .