tailieunhanh - Báo cáo y học: "Zebrafish promoter microarrays identify actively transcribed embryonic genes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Zebrafish promoter microarrays identify actively transcribed embryonic genes. | Method Open Access Zebrafish promoter microarrays identify actively transcribed embryonic genes Fiona C Wardle Duncan T Odom George W BelT Bingbing Yuan Timothy W Danford Elizabeth L Wiellette Elizabeth Herbolsheimer Hazel L Sive Richard A Young and James C Smith Addresses Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology Cambridge University Cambridge CB2 1QN UK. Whitehead Institute for Biomedical Research Nine Cambridge Center Cambridge MA 02142 USA. Computer Science and Artificial Intelligence Laboratory Massachusetts Institute of Technology Vassar Street Cambridge MA 02139 USA. Novartis Institutes for Biomedical Research Mass Ave Cambridge MA 02139 USA. Correspondence FionaCWardle. Email fcw27@ Published 04 August 2006 Genome Biology 2006 7 R71 doi l86 gb-2006-7-8-r7l The electronic version of this article is the complete one and can be found online at http 2006 7 8 R71 Received 11 April 2006 Revised 23 April 2006 Accepted 4 August 2006 2006 Wardle et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11 000 zebrafish genes. Using these microarrays together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3 we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis. Background As the development of an organism proceeds from the fertilized egg to multicellular embryo .