tailieunhanh - Báo cáo y học: "Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons. | Open Access Research Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons Qian-fei WangH n Shyam PrabhakarH Sumita Chanan Jan- Fang Cheng Edward M Rubin and Dario Boffelli Addresses Genomics Division Lawrence Berkeley National Laboratory Berkeley California 94720 USA. tUS Department of Energy Joint Genome Institute Walnut Creek California 94598 USA. Current address Section of Hematology Oncology Department of Medicine University of Chicago 5814 S. Ellis Avenue Chicago IL 60637 USA. n These authors contributed equally to this work. Correspondence Qian-fei Wang. Email qwang2@. Dario Boffelli. Email DBoffelli@ Published 3 January 2007 Genome Biology 2007 8 R1 doi gb-2007-8-1-r1 The electronic version of this article is the complete one and can be found online at http 2007 8 1 R1 Received 2 September 2006 Revised 15 November 2006 Accepted 3 January 2007 2007 Wang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genomic comparisons between human and distant non-primate mammals are commonly used to identify c s-regulatory elements based on constrained sequence evolution. However these methods fail to detect functional elements that are too weakly conserved among mammals to distinguish them from non-functional DNA. Results To evaluate a strategy for large scale genome annotation that is complementary to the commonly used distal species comparisons we explored the potential of deep intra-primate sequence comparisons. We sequenced the orthologs of 558 kb of human genomic sequence covering multiple loci involved in cholesterol homeostasis in 6 non-human primates. Our analysis identified six non-coding DNA .