tailieunhanh - Báo cáo y học: "Fine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Fine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura. | Research Open Access Fine scale structural variants distinguish the genomes of Drosophila melanogaster and D. pseudoobscura Stuart J Macdonald and Anthony D Long Address Department of Ecology and Evolutionary Biology University of California Irvine CA 92697-2525 USA. Correspondence StuartJ Macdonald. Email sjm@ Published 27 July 2006 Genome Biology 2006 7 R67 doi 186 gb-2006-7-7-r67 The electronic version of this article is the complete one and can be found online at http 2006 7 7 R67 Received 7 March 2006 Revised 30 April 2006 Accepted 27 July 2006 2006 Macdonald and Long licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A primary objective of comparative genomics is to identify genomic elements of functional significance that contribute to phenotypic diversity. Complex changes in genome structure insertions duplications rearrangements translocations may be widespread and have important effects on organismal diversity. Any survey of genomic variation is incomplete without an assessment of structural changes. Results We re-examine the genome sequences of the diverged species Drosophila melanogaster and D. pseudoobscura to identify fine-scale structural features that distinguish the genomes. We detect 95 large insertion deletion events that occur within the introns of orthologous gene pairs the majority of which represent insertion of transposable elements. We also identify 143 microinversions below 5 kb in size. These microinversions reside within introns or just upstream or downstream of genes and invert conserved DNA sequence. The sequence conservation within microinversions suggests they may be enriched for functional genetic elements and their position with respect to .