tailieunhanh - Báo cáo y học: "Phylogenetically widespread alternative splicing at unusual GYNGYN donors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Phylogenetically widespread alternative splicing at unusual GYNGYN donors. | Open Access Research Phylogenetically widespread alternative splicing at unusual GYNGYN donors Michael Hiller Klaus Huse Karol Szafranski Philip Rosenstiel Stefan Schreiber Rolf Backofen and Matthias Platzer Addresses Institute of Computer Science Chair for Bioinformatics Albert-Ludwigs-University Freiburg Georges-Koehler-Allee 106 79110 Freiburg Germany. Genome Analysis Leibniz Institute for Age Research - Fritz Lipmann Institute Beutenbergstr. 11 07745 Jena Germany. Institute of Clinical Molecular Biology Christian-Albrechts-University Kiel Schittenhelmstr. 12 24105 Kiel Germany. Max Planck Institute for Molecular Genetics Ihnestr. 63 14195 Berlin Germany. Correspondence Matthias Platzer. Email mplatzer@ Published 25 July 2006 Genome Biology 2006 7 R65 doi 186 gb-2006-7-7-r65 The electronic version of this article is the complete one and can be found online at http 2006 7 7 R65 Received 3 April 2006 Revised 19 May 2006 Accepted 25 July 2006 2006 Hiller et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Splice donor sites have a highly conserved GT or GC dinucleotide and an extended intronic consensus sequence GTRAGT that reflects the sequence complementarity to the U1 snRNA. Here we focus on unusual donor sites with the motif GYNGYN Y stands for C or T N stands for A C G or T . Results While only one GY functions as a splice donor for the majority of these splice sites in human we provide computational and experimental evidence that 110 allow alternative splicing at both GY donors. The resulting splice forms differ in only three nucleotides which results mostly in the insertion deletion of one amino acid. However we also report the insertion of a stop codon

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