tailieunhanh - Báo cáo khoa học: "Developing an Agrobacterium-mediated transformation system for Lilium x formolongo using thin cell layer of bulb scales"

Tuyển tập báo cáo nghiên cứu khoa học của trường đại học nông nghiệp 1 đề tài: Developing an Agrobacterium-mediated transformation system for Lilium x formolongo using thin cell layer of bulb scales. | Journal of Science and Development April 2008 123-128 HANOI UNIVERSITY OF AGRICULTURE DEVELOPING AN AGRORACTERIUMMEUIATEU TRANSFORMATION SySTEM FOR LILIUMX FORMOLONGOUSING THIN CELL LAyER OF BULB SCALES Nguyen Thi Phuong Thao Nguyen Thi Thuy Nguyen Quang Thach Faculty of Agronomy Hanoi University of Agriculture ABSTRACT An Agrobacterium-mediated transformation system for Lilium x formolongo a new lily variety with white flowers and high commercial value has been developed. In vitro bulb scales were sliced into thin layers of 2 mm thick and were cultured on MS medium 1mg l IBA 6 sucrose for about 6 weeks in the dark at 250C to induce callus or bulblets. These explants were then inoculated and co-cultivated for 7 days with a cell suspension of A. tumefaciens strain EHA105 harboring the plasmid plITB02 containing the nptll gene for kanamycin resistance driven by a nos promoter and GUS gene coding for B-glucuronidase and the bar gene coding for phosphinothricin acetyltransferaza. Cefotaxime at 500 mg l and PPT at mg l showed to be the most optimal selection concentrations. Putative transformants which regenerated on selection media were subject to a GUS assay. GUS assay analysis showed 4 of the explants expressing GUS. The transformation system discussed in the present study allows the introduction of useful genes in the Lilium x formolongo genome. Key words Agrobacterium Lilium x formolongo GUS assay transformation bulb scales thin cell layers 1. INTRODUCTION The lily is one of the most important crops in the world flower bulb industry. In Viet nam lilies are the most important ornamental crop. The return from lily cut flowers are 10 to 15 times higher than from chrysanthemum rose carnation cala lily just after orchids . However lily production and commerce in Vietnam has not been developed despite its great potential. There is almost no research programe on lily breeding that have been conducted in the country. In the world a wide range of researche has been .