tailieunhanh - Báo cáo y học: "An all-round view of eukaryotic transcription"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: An all-round view of eukaryotic transcription. | Meeting report An all-round view of eukaryotic transcription Tae Hoon Kim and Bing Ren Addresses Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine University of California San Diego School of Medicine 9500 Gilman Drive La Jolla CA 92093-0653 USA. Correspondence Bing Ren. Email biren@ Published 28 July 2006 Genome Biology 2006 7 323 doi gb-2006-7-7-323 The electronic version of this article is the complete one and can be found online at http 2006 7 7 323 2006 BioMed Central Ltd A report of the Keystone Symposium Regulation of Eukaryotic Transcription From Chromatin to mRNA Taos USA 21-26 April 2006. Transcription of protein-coding genes in eukaryotes involves a complicated yet highly coordinated series of events involving chromatin chromatin modifiers the transcriptional machineries and transcriptional regulators. A recent Keystone Symposium on the regulation of eukaryotic transcription covered the topic from a variety of perspectives both structural and biochemical. This report highlights some of the findings and new approaches reported at the meeting. Structural views of the transcriptional complex One key to understanding the mechanism of transcriptional initiation is an atomic-level view of the RNA polymerase preinitiation complex PIC . In his keynote address Roger Kornberg Stanford University Palo Alto USA described new structural studies of a PIC containing the 12-subunit yeast RNA polymerase II PolII and general transcription factors bound to promoter DNA. From this we can see that TATA binding protein TBP configures DNA to the PolII surface transcription factor II B TFIIB directs the DNA to the PolII active site and stabilizes the transcription complex TFIIE recognizes the closed PolII complex and recruits the helicase TFIIH while TFIIF captures the template strand DNA when the DNA duplex melts to form the transcriptional bubble. Finally TFIIH introduces negative supercoiling of the .