tailieunhanh - Báo cáo y học: "Comparative profiling of the sense and antisense transcriptome of maize lines"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Comparative profiling of the sense and antisense transcriptome of maize lines. | Research Open Access Comparative profiling of the sense and antisense transcriptome of maize lines Jiong Ma Darren J Morrow John Fernandes and Virginia Walbot Address Department of Biological Sciences Stanford University Stanford CA 94305-5020 USA. Correspondence Virginia Walbot. Email walbot@ Published 13 March 2006 Genome Biology 2006 7 R22 doi 186 gb-2006-7-3-r22 The electronic version of this article is the complete one and can be found online at http 2006 7 3 r22 Received 2 November 2005 Revised 13 January 2006 Accepted 8 February 2006 2006 Ma et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background There are thousands of maize lines with distinctive normal as well as mutant phenotypes. To determine the validity of comparisons among mutants in different lines we first address the question of how similar the transcriptomes are in three standard lines at four developmental stages. Results Four tissues leaves 1 mm anthers mm anthers pollen from one hybrid and one inbred maize line were hybridized with the W23 inbred on Agilent oligonucleotide microarrays with 21 000 elements. Tissue-specific gene expression patterns were documented with leaves having the most tissue-specific transcripts. Haploid pollen expresses about half as many genes as the other samples. High overlap of gene expression was found between leaves and anthers. Anther and pollen transcript expression showed high conservation among the three lines while leaves had more divergence. Antisense transcripts represented about 6 to 14 percent of total transcriptome by tissue type but were similar across lines. Gene Ontology GO annotations were assigned and tabulated. Enrichment in GO terms related to .

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