tailieunhanh - Báo cáo y học: "Targeting Drosophila eye development"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Targeting Drosophila eye development. | Minireview Targeting Drosophila eye development Jennifer Jemc and Ilaria Rebay Addresses Department of Biology Massachusetts Institute of Technology Cambridge MA 02142 USA. fBen May Institute for Cancer Research University of Chicago 929 E. 57th Street Chicago IL 60637 USA. Correspondence Ilaria Rebay. Email irebay@ Published 27 July 2006 Genome Biology 2006 7 226 doi gb-2006-7-7-226 The electronic version of this article is the complete one and can be found online at http 2006 7 7 226 2006 BioMed Central Ltd Abstract In order to understand the role of transcription factors in particular developmental processes it is necessary to know their target genes. A combination of bioinformatics comparative expression profiling and microarray-based epistasis experiments has recently identified new targets of Eyeless a key transcription factor in Drosophila retinal determination. The Drosophila retinal determination gene network RDGN consists of seven transcription factors conserved from flies to humans that cooperate to regulate cell specification and determination during eye development. At the top of this network are the master regulators Twin of Eyeless Toy and Eyeless Ey homologs of vertebrate Pax6 which activate expression of the genes for the conserved downstream transcription factors Eyes Absent Eya Sine Oculis So Optix and Dachshund Dac Figure 1 . The transcription factor Eyegone Eyg is also a retinal determination protein but has not yet been positioned within the pathway. At present only a small number of direct targets of any of these transcription factors have been identified see Figure 1 . An elegant strategy integrating bioinformatics and microarraybased expression profiling with in vivo genetics has recently been used by Ostrin et al. 1 to isolate and validate transcriptional targets of Ey. Bringing genetics to genomics microarray epistasis The strength of a large-scale screen lies in its design and in the implementation .