tailieunhanh - Báo cáo y học: "Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes. | Method Open Access Systematic profiling of cellular phenotypes with spotted cell microarrays reveals mating-pheromone response genes Rammohan NarayanaswamyH Wei NiuH Alexander D ScourasH G Traver Hart Jonathan Davies Andrew D Ellington Vishwanath R Iyer and Edward M Marcotte Address Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology 2500 Speedway University of Texas Austin TX 78712 USA. H These authors contributed equally to this work. Correspondence Vishwanath R Iyer. Email vishy@. Edward M Marcotte. Email marcotte@ Published 31 January 2006 Genome Biology 2006 7 R6 doi gb-2006-7-1-r6 The electronic version of this article is the complete one and can be found online at http 2006 7 1 R6 Received 25 July 2005 Revised 14 October 2005 Accepted l0 January 2006 2006 Narayanaswamy et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed stained for subcellular features then imaged via automated high-throughput microscopy allowing systematic phenotypic characterization. We used this technology to identify genes involved in the response of yeast to mating pheromone. Besides morphology assays cell microarrays should be valuable for high-throughput in situ hybridization and immunoassays enabling new classes of genetic assays based on cell imaging. Background A major goal in functional genomics proteomics and systems biology is to define the biological functions of the genes encoded in each genome and to reconstruct the network of functional interactions that underlies normal and altered cellular .