tailieunhanh - Báo cáo y học: "Variable window binding for mutually exclusive alternative splicing"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Variable window binding for mutually exclusive alternative splicing. | Research Open Access Variable window binding for mutually exclusive alternative splicing Dimitris Anastassiou Hairuo Liu and Vinay Varadan Address Center for Computational Biology and Bioinformatics and Department of Electrical Engineering Columbia University New York NY 07670 USA. Correspondence Dimitris Anastassiou. Email anastas@ Published 13 January 2006 Genome Biology 2006 7 R2 doi gb-2006-7-1-r2 The electronic version of this article is the complete one and can be found online at http 2006 7 1 R2 Received 28 September 2005 Revised 21 November 2005 Accepted l6 December 2005 2006 Anastassiou et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Genes of advanced organisms undergo alternative splicing which can be mutually exclusive in the sense that only one exon is included in the mature mRNA out of a cluster of alternative choices often arranged in a tandem array. In many cases however the details of the underlying biologic mechanisms are unknown. Results We describe variable window binding - a mechanism used for mutually exclusive alternative splicing by which a segment window of a conserved nucleotide anchor sequence upstream of the exon 6 cluster in the pre-mRNA of the fruitfly Dscam gene binds to one of the introns thereby activating selection of the exon directly downstream from the binding site. This mechanism is supported by the fact that the anchor sequence can be inferred solely from a comparison of the intron sequences using a genetic algorithm. Because the window location varies for each exon choice regulation can be achieved by obstructing part of that sequence. We also describe a related mechanism based on competing pre-mRNA stem-loop .

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