tailieunhanh - Báo cáo y học: "A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones. | Research Open Access A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones Clare Gooding Francis ClarkHt Matthew C Wollerton Sushma-Nagaraja Grellscheid Harriet Groom and Christopher WJ Smith Addresses Department of Biochemistry University of Cambridge 80 Tennis Court Road Cambridge CB2 1GA UK. ỶAdvanced Computational Modelling Centre and ARC Centre for Bioinformatics University of Queensland Australia. H These authors contributed equally to this work. Correspondence ChristopherWJ Smith. Email cwjsi@ Published 13 January 2006 Genome Biology 2006 7 R1 doi gb-2006-7-1-r1 The electronic version of this article is the complete one and can be found online at http 2006 7 1 R1 Received 26 July 2005 Revised 21 September 2005 Accepted 28 November 2005 2006 Gooding et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The three consensus elements at the 3 end of human introns - the branch point sequence the polypyrimidine tract and the 3 splice site AG dinucleotide - are usually closely spaced within the final 40 nucleotides of the intron. However the branch point sequence and polypyrimidine tract of a few known alternatively spliced exons lie up to 400 nucleotides upstream of the 3 splice site. The extended regions between the distant branch points dBPs and their 3 splice site are marked by the absence of other AG dinucleotides. In many cases alternative splicing regulatory elements are located within this region. Results We have applied a simple algorithm based on AG dinucleotide exclusion zones AGEZ to a large data set of verified human exons. We found a substantial number of exons with large AGEZs which represent