tailieunhanh - Báo cáo y học: "The elusive yeast interactome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The elusive yeast interactome. | Minireview The elusive yeast interactome Johannes Goll and Peter Uetz Addresses Institut fur Genetik Forschungszentrum Karlsruhe Box 3640 76021 Karlsruhe Germany. The Institute of Genomic Research 9712 Medical Center Drive Rockville MD 20850 USA. Correspondence Peter Uetz. Email peter@ Published 30 June 2006 Genome Biology 2006 7 223 doi gb-2006-7-6-223 The electronic version of this article is the complete one and can be found online at http 2006 7 6 223 2006 BioMed Central Ltd Abstract Simple eukaryotic cells such as yeast could contain around 800 protein complexes as two new comprehensive studies show. But slightly different approaches resulted in surprising differences between the two datasets showing that more work is required to get a complete picture of the yeast interactome. Protein complexes are the workhorses of the cell as they are involved in almost all biological processes from transmembrane signaling to gene expression. Only a few are really well understood in terms of structure and function however and many appear to be involved in processes we do not know much about. In two independent recent papers 1 2 groups from the European Molecular Biology Laboratory EMBL Cellzome a spin-off company from EMBL and the University of Toronto have published comprehensive surveys of all the protein complexes detected in yeast - the yeast interac-tome or complexome as one might now call it Table 1 see also 3 4 . This is a landmark achievement given that no other cell or organism has been surveyed at such a level of detail. More important yeast is a prototypic eukaryotic cell that is a model for human cells and most yeast complexes probably have homologs in humans. From proteome to complexome The characterization of protein complexes sounds trivial insert a piece of DNA encoding a tag into a protein-coding gene and let the cells express the tagged protein the bait . Then break up the cell and pull out the tagged protein with all its .