tailieunhanh - Báo cáo y học: "Large-scale and high-confidence proteomic analysis of human seminal plasma"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Large-scale and high-confidence proteomic analysis of human seminal plasma. | Research Open Access Large-scale and high-confidence proteomic analysis of human seminal plasma Bartosz Pilch and Matthias Mann Addresses Center for Experimental BioInformatics CEBI Department of Biochemistry and Molecular Biology University of Southern Denmark Campusvej 55 DK-5230 Odense M Denmark. ỶDepartment of Proteomics and Signal Transduction Max Planck Institute for Biochemistry Am Klopferspitz 18 D-82152 Martinsried Germany. Correspondence Matthias Mann. Email mmann@ Published 18 May 2006 Genome Biology 2006 7 R40 doi 186 gb-2006-7-5-r40 The electronic version of this article is the complete one and can be found online at http 2006 7 5 R40 Received 16 November 2005 Revised 13 December 2005 Accepted 10 April 2006 2006 Pilch and Mann licensee BioMed Central Ltd This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The development of mass spectrometric MS techniques now allows the investigation of very complex protein mixtures ranging from subcellular structures to tissues. Body fluids are also popular targets of proteomic analysis because of their potential for biomarker discovery. Seminal plasma has not yet received much attention from the proteomics community but its characterization could provide a future reference for virtually all studies involving human sperm. The fluid is essential for the survival of spermatozoa and their successful journey through the female reproductive tract. Results Here we report the high-confidence identification of 923 proteins in seminal fluid from a single individual. Fourier transform MS enabled parts per million mass accuracy and two consecutive stages of MS fragmentation allowed confident identification of proteins even by single peptides. Analysis with .