tailieunhanh - Báo cáo y học: "The undertranslated transcriptome reveals widespread translational silencing by alternative 5' transcript leaders"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The undertranslated transcriptome reveals widespread translational silencing by alternative 5' transcript leaders. | Research Open Access The undertranslated transcriptome reveals widespread translational silencing by alternative 5 transcript leaders G Lynn Law Kellie S Bickel Vivian L MacKay and David R Morris Address Department of Biochemistry University of Washington Seattle WA 98195 USA. H These authors contributed equally to this work. Correspondence DavidRMorris. Email dmorris@ Published 3 January 2006 Genome Biology 2005 6 R1 I I doi gb-2005-6-13-r111 The electronic version of this article is the complete one and can be found online at http 2005 6 13 R1 11 Received 2 September 2005 Revised 17 October 2005 Accepted 21 November 2005 2005 Law et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Translational efficiencies in Saccharomyces cerevisiae vary from transcript to transcript by approximately two orders of magnitude. Many of the poorly translated transcripts were found to respond to the appropriate external stimulus by recruiting ribosomes. Unexpectedly a high frequency of these transcripts showed the appearance of altered 5 leaders that coincide with increased ribosome loading. Results Of the detectable transcripts in S. cerevisiae 8 were found to be underloaded with ribosomes. Gene ontology categories of responses to stress or external stimuli were overrepresented in this population of transcripts. Seventeen poorly loaded transcripts involved in responses to pheromone nitrogen starvation and osmotic stress were selected for detailed study and were found to respond to the appropriate environmental signal with increased ribosome loading. Twelve of these regulated transcripts exhibited structural changes in their 5 transcript leaders in response to the environmental