tailieunhanh - Báo cáo y học: "The regulation of endocytosis by kinases: cell biology meets genomic"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: The regulation of endocytosis by kinases: cell biology meets genomics. | Minireview The regulation of endocytosis by kinases cell biology meets genomics Zita Balklava and Barth D Grant Address Department of Molecular Biology and Biochemistry Rutgers University Piscataway NJ 08854 USA. Correspondence Barth D Grant. E-mail grant@ Published 3 January 2006 Genome Biology 2005 6 245 doi gb-2005-6-l3-245 The electronic version of this article is the complete one and can be found online at http 2005 6 13 245 2005 BioMed Central Ltd Abstract The mechanisms of signal transduction and vesicular transport have traditionally been studied in isolation but recent studies make it clear that the two processes are inextricably linked. A new genome-wide analysis of human kinases using RNA interference shows an unexpected depth and complexity to the interactions between these processes. Since the discovery in the nematode Caenorhabditis elegans that the introduction of a double-stranded RNA dsRNA trigger can lead to the selective inhibition of gene expression in a sequence-specific manner 1 this phenomenon - now widely known as RNA interference RNAi - has spawned many new applications. Among these is the invention of a whole area of genomics research dedicated to studying RNAi-induced phenotypes. Such phenotypes generally mimic reduction-of-function or loss-of-function mutations that have formed the backbone of traditional genetics research for more than a century. Most large-scale screens using this method have been performed in model organisms such as C. elegans 2-4 and Drosophila melanogaster 5 6 . As a result of recent insights into the mechanism of RNAi and the resulting identification of small interfering RNAs siRNAs such large-scale approaches are now also feasible in cultured mammalian cells 7 8 . The quantity of data derived from large-scale or whole-genome RNAi-based screens while sometimes overwhelming has begun to provide insights into complex biological phenomena that had previously proved .