tailieunhanh - Báo cáo y học: "Canonical Wnt signaling: high-throughput RNAi widens the path"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Canonical Wnt signaling: high-throughput RNAi widens the path. | Minireview Canonical Wnt signaling high-throughput RNAi widens the path Anthony MC Brown Addresses Department of Cell and Developmental Biology Weill Medical College of Cornell University and Strang Cancer Prevention Center New York NY 10021 USA. E-mail amcbrown@ Published 31 August 2005 Genome Biology 2005 6 231 doi gb-2005-6-9-231 The electronic version of this article is the complete one and can be found online at http 2005 6 9 231 2005 BioMed Central Ltd Abstract The canonical Wnt signaling pathway is highly conserved in evolution widely used throughout animal development and frequently hyperactive in cancer. Although Wnt signaling has been the subject of extensive genetic analysis in the past some 200 genes have now been identified as candidate modulators of this pathway by a recent study using high-throughput RNAi screening. The discovery of small interfering RNAs siRNAs in conjunction with whole-genome sequence data has spawned a 21st-century strategy for carrying out surrogate genetics on a grand scale - high-throughput RNA interference RNAi analysis 1 2 . Using libraries of siRNAs that target potentially all transcripts one can screen for the phenotypic effects of knocking down individual genes on a genome-wide scale. Perhaps more than any other technique high-throughput RNAi encapsulates the full meaning of functional genomics. DasGupta and colleagues 3 have recently described the use of such screening for identifying genes that modulate one of the major signal transduction mechanisms in animal cells the Wnt p-catenin pathway. Wnt proteins activate one of the most frequently encountered intracellular signaling pathways in all of developmental biology one that has innumerable roles in the development of multicellular animals from sea anemones to humans 4 . The Wnt family of secreted signaling factors is also one of the most ancient pre-dating the divergence of cnidarians and bilateral metazoans 650 million years ago