tailieunhanh - Báo cáo y học: "Genomic analysis of heat-shock factor targets in Drosophila"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genomic analysis of heat-shock factor targets in Drosophila. | Method Open Access Genomic analysis of heat-shock factor targets in Drosophila Ian Birch-MachinH Shan GaoHt David Huen Richard McGirr Robert AH White and Steven Russelh Addresses Department of Anatomy University of Cambridge Downing Street Cambridge CB2 3EH UK. ỶDepartment of Genetics University of Cambridge Downing Street Cambridge CB2 3EH UK. H These authors contributed equally to this work. Correspondence Steven Russell. E-mail Published 10 June 2005 Genome Biology 2005 6 R63 doi 186 gb-2005-6-7-r63 The electronic version of this article is the complete one and can be found online at http 2005 6 7 R63 Received 31 January 2005 Revised 7 April 2005 Accepted 10 May 2005 2005 Birch-Machin et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have used a chromatin immunoprecipitation-microarray ChIP-array approach to investigate the in vivo targets of heat-shock factor Hsf in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms. Background Chromatin immunoprecipitation or more correctly immunopurification ChIP has emerged as a valuable approach for identifying the in vivo binding sites of transcription factors 1-6 . Before the availability of complete genome sequence the use of this approach for identifying transcription targets on a genome-wide scale was however limited. Over the past few years a number of laboratories have successfully used .