tailieunhanh - Báo cáo y học: "Genomic analysis of metabolic pathway gene expression in mice"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genomic analysis of metabolic pathway gene expression in mice. | Research Open Access Genomic analysis of metabolic pathway gene expression in mice Anatole Ghazalpour Sudheer Doss Sonal S Sheth Leslie A IngramDrake Eric E Schadt Aldons J Lusis and Thomas A Drake Addresses Department of Human Genetics Department of Medicine and Department of Microbiology Immunology and Molecular Genetics and Molecular Biology Institute University of California Los Angeles CA 90095-1679 USA. Department of Pathology and Laboratory Medicine University of California Los Angeles CA 90095-1732 USA. Rosetta Inpharmatics LLC Kirkland WA 98034 USA. Correspondence ThomasADrake. E-mail tdrake@ Published I July 2005 Genome Biology 2005 6 R59 doi 186 gb-2005-6-7-r59 The electronic version of this article is the complete one and can be found online at http 2005 6 7 R59 Received 8 December 2004 Revised 1 February 2005 Accepted 8 June 2005 2005 Ghazalpour et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background A segregating population of C57BL 6J X DBA 2J F2 intercross mice was studied for obesity-related traits and for global gene expression in liver. Quantitative trait locus analyses were applied to the subcutaneous fat-mass trait and all gene-expression data. These data were then used to identify gene sets that are differentially perturbed in lean and obese mice. Results We integrated global gene-expression data with phenotypic and genetic segregation analyses to evaluate metabolic pathways associated with obesity. Using two approaches we identified 13 metabolic pathways whose genes are coordinately regulated in association with obesity. Four genomic regions on chromosomes 3 6 16 and 19 were found to control the coordinated expression of these pathways. Using .