tailieunhanh - Báo cáo y học: "he discovery, positioning and verification of a set of transcription-associated motifs in vertebrates"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The discovery, positioning and verification of a set of transcription-associated motifs in vertebrates. | Method Open Access The discovery positioning and verification of a set of transcription-associated motifs in vertebrates Laurence EttwillerH Benedict PatenH Marcel SourenHt Felix LooslL Jochen WittbrodU and Ewan Birney Addresses EBI Wellcome Trust Genome Campus Hinxton Cambridgeshire CB10 1SD UK. ỶEMBL Meyerhofstrasse 69012 Heidelberg Germany. H These authors contributed equally to this work. Correspondence Ewan Birney. E-mail birney@ Published 2 December 2005 Genome Biology 2005 6 Rl04 doi gb-2005-6-l2-rl04 The electronic version of this article is the complete one and can be found online at http 2005 6 l2 Rl04 Received 22 August 2005 Revised 18 October 2005 Accepted 8 November 2005 2005 Ettwiller et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have developed several new methods to investigate transcriptional motifs in vertebrates. We developed a specific alignment tool appropriate for regions involved in transcription control and exhaustively enumerated all possible l2-mers for involvement in transcription by virtue of their mammalian conservation. We then used deeper comparative analysis across vertebrates to identify the active instances of these motifs. We have shown experimentally in Medaka fish that a subset of these predictions is involved in transcription. Background A genome encodes more than just the structural proteins or RNA sequences that form active biological molecules. In addition the control of expression of these structural genes is determined by elements that act at the DNA RNA or epigenetic level and are associated with specific genes in some manner. Considerable knowledge of these regulatory networks is available for specific sets of genes for .