tailieunhanh - Báo cáo y học: "Biased alternative polyadenylation in human tissues"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Biased alternative polyadenylation in human tissues. | Research Biased alternative polyadenylation in human tissues Haibo Zhang Ju Youn Lee and Bin Tian Open Access Address Department of Biochemistry and Molecular Biology New Jersey Medical School University of Medicine and Dentistry of New Jersey 185 South Orange Avenue Newark NJ 07101-1709 USA. Correspondence Bin Tian. E-mail btian@ Published 28 November 2005 Genome Biology 2005 6 Rl00 doi gb-2005-6-l2-rl00 The electronic version of this article is the complete one and can be found online at http 2005 6 l2 Rl00 Received 13 June 2005 Revised 3 l August 2005 Accepted l8 October 2005 2005 Zhang et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Alternative polyadenylation is one of the mechanisms in human cells that give rise to a variety of transcripts from a single gene. More than half of the human genes have multiple polyadenylation sites poly A sites leading to variable mRNA and protein products. Previous studies of individual genes have indicated that alternative polyadenylation could occur in a tissuespecific manner. Results We set out to systematically investigate the occurrence and mechanism of alternative polyadenylation in different human tissues using bioinformatic approaches. Using expressed sequence tag EST data we investigated 42 distinct tissue types. We found that several tissues tend to use poly A sites that are biased toward certain locations of a gene such as sites located in introns or internal exons and various sites in the exon located closest to the 3 end. We also identified several tissues including eye retina and placenta that tend to use poly A sites not frequently used in other tissues. By exploring microarray expression data we analyzed over .