tailieunhanh - Báo cáo y học: "A computational investigation of kinetoplastid trans-splicing"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: A computational investigation of kinetoplastid trans-splicing. | Method Open Access A computational investigation of kinetoplastid trans-splicing Shuba Gopal Saria Awadalla Terry Gaasterland and George AM Cross Addresses Laboratory of Computational Genomics Rockefeller University 1230 York Avenue New York NY 10021 USA. Department of Biological Sciences Rochester Institute of Technology 85 Lomb Memorial Drive Rochester NY 14623 USA. Laboratory of Molecular Parasitology Rockefeller University 1230 York Avenue New York NY 10021 USA. Correspondence Shuba Gopal. E-mail sxgsbi@ Published 17 October 2005 Genome Biology 2005 6 R95 doi l86 gb-2005-6-l 1-r95 The electronic version of this article is the complete one and can be found online at http 2005 6 ll R95 Received 23 May 2005 Revised 28 July 2005 Accepted 7 September 2005 2005 Gopal et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Trans-splicing is an unusual process in which two separate RNA strands are spliced together to yield a mature mRNA. We present a novel computational approach which has an overall accuracy of 82 and can predict 92 of known trans-splicing sites. We have applied our method to chromosomes l and 3 of Leishmania major with high-confidence predictions for 85 and 88 of annotated genes respectively. We suggest some extensions of our method to other systems. Background RNA splicing is a key process in the transformation of genomic instructions into functional proteins and may play a critical role in regulating gene expression in a variety of eukaryotes. Two forms of splicing have been documented in eukaryotes. Many eukaryotes use cis-splicing the process of removing introns from precursor RNAs to generate mature mRNAs. A related and less understood process trans-splic-ing appears