tailieunhanh - Báo cáo y học: "Weighting by heritability for detection of quantitative trait loci with microarray estimates of gene expression"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Weighting by heritability for detection of quantitative trait loci with microarray estimates of gene expression. | Method Open Access Weighting by heritability for detection of quantitative trait loci with microarray estimates of gene expression Kenneth F Manly Jintao Wang and Robert W Williams Addresses Department of Pathology University of Tennessee Health Science Center 855 Monroe Avenue Memphis TN 38163 USA. Department of Anatomy and Neurobiology Center of Excellence in Genomics and Bioinformatics University of Tennessee Health Science Center 855 Monroe Avenue Memphis TN 38163 USA. Department of Biostatistics 246 Farber Hall University at Buffalo Buffalo NY 14214 USA. Correspondence Kenneth F Manly. E-mail kmanly@ Published 28 February 2005 Genome Biology 2005 6 R27 The electronic version of this article is the complete one and can be found online at http 2005 6 3 R27 Received 25 November 2004 Revised 26 January 2005 Accepted 16 February 2005 2005 Manly et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Heritable differences in transcribed RNA levels can be mapped as quantitative trait loci QTLs . Transcribed RNA levels are often measured by hybridization to microarrays of oligonucleotide probes in which each transcript is represented by multiple probes. The use of recombinant inbred lines allows an estimate of the heritability of expression measured by individual probes. This heritability varies greatly. We have tested heritability-weighted averages to define expression of a transcript and found that these allow detection of more QTLs than previously described methods. Background The steady-state abundance of an RNA species in an organ is in part genetically controlled and can be considered a quantitative genetic trait. Microarray methods for estimating RNA sequence abundance 1 combined .