tailieunhanh - Báo cáo y học: "Serendipitous discovery of Wolbachia genomes in multiple Drosophila species"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Serendipitous discovery of Wolbachia genomes in multiple Drosophila species. | Research Open Access Serendipitous discovery of Wolbachia genomes in multiple Drosophila species Steven L Salzberg Julie C Dunning Hotopp Arthur L Delcher Mihai Pop Douglas R Smith Michael B Eisen and William C Nelson Addresses The Institute for Genomic Research 9712 Medical Center Drive Rockville MD 20850 USA. Agencourt Bioscience Corporation 100 Cumming Center Beverley MA 01915 USA. Center for Integrative Genomics University of California Berkeley CA 94720 USA. Correspondence Steven L Salzberg. E-mail salzberg@ Published 22 February 2005 Genome Biology 2005 6 R23 The electronic version of this article is the complete one and can be found online at http 2005 6 3 R23 Received 22 December 2004 Revised 24 January 2005 Accepted 24 January 2005 2005 Salzberg et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The Trace Archive is a repository for the raw unanalyzed data generated by large-scale genome sequencing projects. The existence of this data offers scientists the possibility of discovering additional genomic sequences beyond those originally sequenced. In particular if the source DNA for a sequencing project came from a species that was colonized by another organism then the project may yield substantial amounts of genomic DNA including near-complete genomes from the symbiotic or parasitic organism. Results By searching the publicly available repository of DNA sequencing trace data we discovered three new species of the bacterial endosymbiont Wolbachia pipientis in three different species of fruit fly Drosophila ananassae D. simulans and D. mojavensis. We extracted all sequences with partial matches to a previously sequenced Wolbachia strain and assembled those sequences .