tailieunhanh - Báo cáo y học: " Ways to get from plant genomes to phenomes: via yeast"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Ways to get from plant genomes to phenomes: via yeast. | Meeting report Ways to get from plant genomes to phenomes via yeast Pablo D Rabinowicz and Willem Rensink Address The Institute for Genomic Research 9712 Medical Center Drive Rockville MD 20850 USA. Correspondence Pablo D Rabinowicz. E-mail pablo@ Published 15 February 2005 Genome Biology 2005 6 310 The electronic version of this article is the complete one and can be found online at http 2005 6 3 3l0 2005 BioMed Central Ltd A report on the Cold Spring Harbor Laboratory meeting Plant Genomes From Sequence to Phenome Cold Spring Harbor USA 9-12 December 2004. Whole-genome sequencing although not yet a routine laboratory technique is certainly becoming more affordable and increasing numbers of nearly complete eukaryote genomes are relentlessly being added to the list. Making sense of the resulting overwhelming amount of sequence may require an equal effort. Several high-throughput tools for automated identification of genes at the structural level are available but functional annotation can only be tentatively inferred on the basis of sequence motifs or sequence similarity. Gold standard structural and functional annotation still requires extensive human intervention to eliminate frequent errors. The next challenge is to investigate how a genome sequence determines the phenotype of the whole organism sometimes referred to as the phenome . The way in which each protein contributes to the phenotype depends on a variety of factors such as regulation of expression interaction with other proteins or nucleic acids response to small molecules subcellular localization and so on. Technologies for genome-wide analysis of gene expression such as microarray hybridization are now commonly used and genome-wide analyses of protein-protein or protein-DNA interactions the interactome are emerging. The complexity of higher eukaryotic genomes makes analysis difficult however particularly for interactomes. This was reflected in a meeting on the functional .