tailieunhanh - Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails. | Method Open Access Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly A tails Hirofumi Kunitomo Hiroko UesugY Yuji KohaiU and Yuichi lino Addresses Molecular Genetics Research Laboratory The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan. tGenome Biology Laboratory National Institute of Genetics Mishima 411-8540 Japan. Correspondence Yuichi lino. E-mail iino@ Published 31 January 2005 Genome Biology 2005 6 R17 The electronic version of this article is the complete one and can be found online at http 2005 6 2 R17 Received 17 September 2004 Revised 29 November 2004 Accepted 21 December 2004 2005 Kunitomo et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract It is not always easy to apply microarray technology to small numbers of cells because of the difficulty in selectively isolating mRNA from such cells. We report here the preparation of mRNA from ciliated sensory neurons of Caenorhabditis elegans using the mRNA-tagging method in which poly A RNA was co-immunoprecipitated with an epitope-tagged poly A -binding protein specifically expressed in sensory neurons. Subsequent cDNA microarray analyses led to the identification of a panel of sensory neuron-expressed genes. Background Recent advances in technologies for analyzing whole-genome gene-expression patterns have provided a wealth of information on the complex transcriptional regulatory networks and changes in gene-expression patterns that are related to phenotypic changes caused by environmental stimuli or genetic alterations. Changes in gene expression are also fundamental during development and cellular differentiation and differences in gene