tailieunhanh - Báo cáo y học: "Variation in tissue-specific gene expression among natural populations"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Variation in tissue-specific gene expression among natural populations. | Open Access Research Variation in tissue-specific gene expression among natural populations Andrew Whitehead and Douglas L Crawford Address Rosenstiel School of Marine and Atmospheric Science University of Miami 4600 Rickenbacker Causeway Miami FL 33149 USA. Correspondence AndrewWhitehead. E-mail awhitehead@ Published 26 January 2005 Genome Biology 2005 6 R1 3 The electronic version of this article is the complete one and can be found online at http 2005 6 2 R13 Received 28 June 2004 Revised 2 September 2004 Accepted 6 December 2004 2005 Whitehead and Crawford licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Variation in gene expression is extensive among tissues individuals strains populations and species. The interactions among these sources of variation are relevant for physiological studies such as disease or toxic stress for example it is common for pathologies such as cancer heart failure and metabolic disease to be associated with changes in tissue-specific gene expression or changes in metabolic gene expression. But how conserved these differences are among outbred individuals and among populations has not been well documented. To address this we examined the expression of a selected suite of 192 metabolic genes in brain heart and liver in three populations of the teleost fish Fundulus heteroclitus using a highly replicated experimental design. Results Half of the genes 48 were differentially expressed among individuals within a population-tissue group and 76 were differentially expressed among tissues. Differences among tissues reflected well established tissue-specific metabolic requirements suggesting that these measures of gene expression accurately reflect .