tailieunhanh - Báo cáo y học: "Function-informed transcriptome analysis of Drosophila renal tubule"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Function-informed transcriptome analysis of Drosophila renal tubule. | Research Open Access Function-informed transcriptome analysis of Drosophila renal tubule Jing Wang Laura Kean Jingli Yang Adrian K Allan Shireen A Davies Pawel Herzyk and Julian AT Dow Addresses Division of Molecular Genetics Institute of Biomedical and Life Sciences University of Glasgow Glasgow G11 6NU UK. ỶSir Henry Wellcome Functional Genomics Facility University of Glasgow Glasgow G12 8QQ UK. Correspondence JulianAT Dow. E-mail Published 26 August 2004 Genome Biology 2004 5 R69 The electronic version of this article is the complete one and can be found online at http 2004 5 9 R69 Received 14 May 2004 Revised 25 June 2004 Accepted 23 July 2004 2004 Wang et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Comprehensive tissue-specific microarray analysis is a potent tool for the identification of tightly defined expression patterns that might be missed in whole-organism scans. We applied such an analysis to Drosophila melanogaster Malpighian renal tubule a defined differentiated tissue. Results The transcriptome of the D. melanogaster Malpighian tubule is highly reproducible and significantly different from that obtained from whole-organism arrays. More than 200 genes are more than 10-fold enriched and over 1 000 are significantly enriched. Of the top 200 genes only 18 have previously been named and only 45 have even estimates of function. In addition 30 transcription factors not previously implicated in tubule development are shown to be enriched in adult tubule and their expression patterns respect precisely the domains and cell types previously identified by enhancer trapping. Of Drosophila genes with close human disease homologs 50 are enriched .