tailieunhanh - Báo cáo y học: "n Ambystoma mexicanum EST sequencing project: analysis of 17,352 expressed sequence tags from embryonic and regenerating blastema cDNA libraries"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: An Ambystoma mexicanum EST sequencing project: analysis of 17,352 expressed sequence tags from embryonic and regenerating blastema cDNA libraries. | Research Open Access An Ambystoma mexicanum EST sequencing project analysis of 17 352 expressed sequence tags from embryonic and regenerating blastema cDNA libraries Bianca Habermann Anne-Gaelle Bebin Stephan Herklotz Michael Volkmer Kay Eckelt Kerstin Pehlke Hans Henning Epperlein Hans Konrad Schackert Glenis Wiebe and Elly M Tanaka Addresses Scionics Computer Innovation GmbH Pfotenhauerstrasse 110 Dresden 01307 Germany. Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 Dresden 01307 Germany. institute of Anatomy Medical Faculty of the Carl Gustav Carus Technical University Dresden Fetscherstrasse 74 Dresden 01307 Germany. Department of Surgical Research Medical Faculty of the Carl Gustav Carus Technical University Dresden Fetscherstrasse 74 Dresden 01307 Germany. Correspondence Bianca Habermann. E-mail habermann@. Elly M Tanaka. E-mail tanaka@ Published 13 August 2004 Genome Biology 2004 5 R67 The electronic version of this article is the complete one and can be found online at http 2004 5 9 R67 Received 17 November 2003 Revised 6 May 2004 Accepted 29 June 2004 2004 Habermann et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The ambystomatid salamander Ambystoma mexicanum axolotl is an important model organism in evolutionary and regeneration research but relatively little sequence information has so far been available. This is a major limitation for molecular studies on caudate development regeneration and evolution. To address this lack of sequence information we have generated an expressed sequence tag EST database for A. mexicanum. Results Two cDNA libraries one made from stage 18-22 embryos and the other from day-6 .