tailieunhanh - Báo cáo y học: "Microarray-based resequencing of multiple Bacillus anthracis isolates"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Microarray-based resequencing of multiple Bacillus anthracis isolates. | Method Open Access Microarray-based resequencing of multiple Bacillus anthracis isolates Michael E Zwick Farrell Mcafee David J Cutler Timothy D Read Jacques Ravel Gregory R Bowman Darrell R Galloway and Alfred Mateczun Addresses Biological Defense Research Directorate Naval Medical Research Center 503 Robert Grant Avenue Silver Spring MD 20910 USA. Department of Human Genetics Emory University School of Medicine Atlanta GA 30322 USA. McKusick-Nathans Institute of Genetic Medicine Johns Hopkins University School of Medicine 733 North Broadway Baltimore MD 21205 USA. The Institute for Genomic Research 9712 Medical Center Drive Rockville MD 20850 USA. Correspondence Michael E Zwick. E-mail mzwick@ Published 17 December 2004 Genome Biology 2004 6 R10 The electronic version of this article is the complete one and can be found online at http 2004 6 1 R10 Received 26 July 2004 Revised 18 October 2004 Accepted 19 November 2004 2004 Zwick et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We used custom-designed resequencing arrays to generate Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication discrepancy rate of X 10-7 and by comparison to independently generated shotgun sequence discrepancy rate X 10-6 . Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains. Background Population genomics the study of genome-wide patterns of genetic variation in a large number of organisms is emerging as a vigorous new field of study 1-3 . Rapid accurate and inexpensive .