tailieunhanh - Báo cáo khoa học: Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination

A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this review, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond solution structure determination. | ẶFEBS Journal REVIEW ARTICLE Macromolecular NMR spectroscopy for the non-spectroscopist beyond macromolecular solution structure determination Michael Bieri1 Ann H. Kwan2 Mehdi Mobli3 Glenn F. King3 Joel P. Mackay2 and Paul R. Gooley1 1 Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Parkville Australia 2 Schoolof Molecular Bioscience The University of Sydney Australia 3 Institute for Molecular Bioscience The University of Queensland St. Lucia Australia Keywords chemical shift mapping NMR NOE protein protein complex protein dynamics protein folding protein interaction protein mutagenesis saturation difference Correspondence P. R. Gooley Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology Institute University of Melbourne Parkville VIC 3010 Australia Fax 61 3 9348 1421 Tel 61 3 8344 2273 E-mail prg@ Received 20 July 2010 revised 7 November 2010 accepted 5 January 2011 doi A strength of NMR spectroscopy is its ability to monitor on an atomic level molecular changes and interactions. In this review which is intended for non-spectroscopists we describe major uses of NMR in protein science beyond solution structure determination. After first touching on how NMR can be used to quickly determine whether a mutation induces structural perturbations in a protein we describe the unparalleled ability of NMR to monitor binding interactions over a wide range of affinities molecular masses and solution conditions. We discuss the use of NMR to measure the dynamics of proteins at the atomic level and over a wide range of timescales. Finally we outline new and expanding areas such as macromolecular structure determination in multicomponent systems as well as in the solid state and in vivo. Introduction As detailed in the accompanying review 1 simple NMR spectra such as the 1D 1H NMR spectrum and 15N-HSQC can rapidly .