tailieunhanh - Báo cáo khoa học: Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-specific Rnf33⁄Trim60 gene

We have previously reported a mouseRnf33⁄Trim60gene that is temporally expressed in the pre-implantation embryo. TheRnf33structural gene is com-posed of a short noncoding exon 1 and an intronless coding exon 2. In the present work,Rnf33was shown to be expressed in the mouse testis and in the testicular cell lines TM3 and TM4. | IFEBS Journal Nuclear factor kappa B and tumor necrosis factor-alpha modulation of transcription of the mouse testis- and pre-implantation development-specific Rnf33 Trim60 gene Kong-Bung Choo1 2 3 Min-Chuan Hsu1 2 Yao-Hui Tsai1 4 Wan-Yi Lin1 and Chiu-Jung Huang4 1 Department of MedicalResearch and Education Taipei Veterans General Hospital Taipei Taiwan 2 Department of Biotechnology and Laboratory Science in Medicine Taipei Veterans General Hospital Taipei Taiwan 3 Institute of ClinicalMedicine NationalYang Ming University Taipei Taiwan 4 Department of AnimalScience and Graduate Institute of Biotechnology Schoolof Agriculture Chinese Culture University Yangmingshan Taipei Taiwan Keywords nuclear factor-kappa B NF-kB p65 and p50 proteins Rnf33 testis-specific gene expression tumor necrosis factor-alfa TNF-a Correspondence Chiu-Jung Huang PhD Associate Professor Department of AnimalScience and Graduate Institute of Biotechnology School of Agriculture Chinese Culture University 55 Hwa-Kang Road Yangmingshan Taipei 111 Taiwan Fax 886 2 28613100 Tel 886 2 28610511 ext. 31231 E-mail hqr2@ Received 29 July 2010 revised 15 November 2010 accepted 24 December 2010 doi We have previously reported a mouse Rnf33 Trim60 gene that is temporally expressed in the pre-implantation embryo. The Rnf33 structural gene is composed of a short noncoding exon 1 and an intronless coding exon 2. In the present work Rnf33 was shown to be expressed in the mouse testis and in the testicular cell lines TM3 and TM4. To elucidate Rnf33 transcriptional modulation a Rnf33 sequence inclusive of the upstream regulatory region exon 1 and the associated intronic sequence was dissected in transient transfection and luciferase assays. An initiator and an atypical TATA-box were shown to act as the core promoter elements of the gene. Deletion and mutagenesis of the sequence in luciferase constructs further demonstrated that an intronic and .