tailieunhanh - Báo cáo y học: "Hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal protein"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal proteins. | Research Open Access Hidden localization motifs naturally occurring peroxisomal targeting signals in non-peroxisomal proteins Georg Neuberger Markus Kunze Frank Eisenhaber Johannes Berger Andreas Hartig and Cecile Brocard Addresses Research Institute of Molecular Pathology IMP Dr Bohr-Gasse 7 A-1030 Vienna Austria. Brain Research Institute Department of Neuroimmunology Medical University Vienna Spitalgasse 4 A-1090 Vienna Austria. Max F Perutz Laboratories Institute of Biochemistry and Molecular Cell Biology University of Vienna and Ludwig-Boltzmann-Forschungsstelle fur Biochemie Dr Bohr-Gasse 9 A-1030 Vienna Austria. Correspondence Frank Eisenhaber. E-mail Published 30 November 2004 Genome Biology 2004 5 R97 The electronic version of this article is the complete one and can be found online at http 2004 5 12 R97 Received 25 May 2004 Revised 11 October 2004 Accepted 9 November 2004 2004 Neuberger et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Can sequence segments coding for subcellular targeting or for posttranslational modifications occur in proteins that are not substrates in either of these processes Although considerable effort has been invested in achieving low false-positive prediction rates even accurate sequence-analysis tools for the recognition of these motifs generate a small but noticeable number of protein hits that lack the appropriate biological context but cannot be rationalized as false positives. Results We show that the carboxyl termini of a set of definitely non-peroxisomal proteins with predicted peroxisomal targeting signals interact with the peroxisomal matrix protein receptor peroxin 5 PEX5 in a yeast two-hybrid test. .