tailieunhanh - Báo cáo khoa học: Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague

Human embryonic stem cells (hESCs) and induced pluripotent stem cells are excellent models for the study of embryonic hematopoiesis in vitro, aiding the design of new differentiation models that may be applicable to cell-replacement therapies. | IFEBS Journal REVIEW ARTICLE Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague Inmaculada Moreno-Gimeno1 Maria H. Ledran1 2 and Majlinda Lako1 2 1 Centro de Investigacion Príncipe Felipe Valencia Spain 2 Institute of Human Genetics Newcastle University InternationalCentre for Life UK Keywords blood cells engraftment hematopoiesis hematopoietic differentiation hematopoietic ontogeny hematopoietic progenitors hESC and iPSC therapeutic applications human embryonic stem cells induced pluripotent stem cells SCID repopulating cells Correspondence I. Moreno-Gimeno Centro de Investigacion Príncipe Felipe Valencia 46012 Spain Fax 00 34 963289701 Tel 00 34 963289680 E-mail imoreno@ Received 22 June 2010 revised 10 September 2010 accepted 18 October 2010 Human embryonic stem cells hESCs and induced pluripotent stem cells are excellent models for the study of embryonic hematopoiesis in vitro aiding the design of new differentiation models that may be applicable to cell-replacement therapies. Adult and fetal hematopoietic stem cells are currently being used in biomedical applications however the latest advances in regenerative medicine and stem cell biology suggest that hESC-derived hematopoietic stem cells are an outstanding tool for enhancing immunotherapy and treatments for blood disorders and cancer for example. In this review we compare various methods used for inducing in vitro hematopoietic differentiation from hESCs based on co-culture with stromal cells or formation of embryoid bodies and analyse their ability to give rise to hematopoietic precursors with emphasis on their engraftment potential as a measure of their functionality in vivo. doi Why use human embryonic stem cells or induced pluripotent stem cells for hematopoiesis studies The first paper .