tailieunhanh - Báo cáo khoa học: Localizing matrix metalloproteinase activities in the pericellular environment

Matrix metalloproteinases (MMPs) are a group of structurally related pro-teolytic enzymes containing a zinc ion in the active site. They are secreted from cells or bound to the plasma membrane and hydrolyze extracellular matrix (ECM) and cell surface-bound molecules. | IFEBS Journal MINIREVIEW Localizing matrix metalloproteinase activities in the pericellular environment Gillian Murphy1 and Hideaki Nagase2 1 Department of Oncology University of Cambridge Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge UK 2 Department of Matrix Biology The Kennedy Institute of Rheumatology Division Faculty of Medicine ImperialCollege London UK Keywords CD44 collagen extracellular matrix integrin proteoglycan receptor tetraspanin Correspondence H. Nagase Department of Matrix Biology The Kennedy Institute of Rheumatology Division Faculty of Medicine Imperial College London London W6 8LH UK Fax 44 02083834488 Tel 02083834994 E-mail Received 26 June 2010 revised 15 September 2010 accepted 22 September 2010 Matrix metalloproteinases MMPs are a group of structurally related proteolytic enzymes containing a zinc ion in the active site. They are secreted from cells or bound to the plasma membrane and hydrolyze extracellular matrix ECM and cell surface-bound molecules. They therefore play key roles in morphogenesis wound healing tissue repair and remodeling in diseases such as cancer and arthritis. Although the cell anchored membranetype MMPs MT-MMPs function pericellularly the secreted MMPs have been considered to act within the ECM away from the cells from which they are synthesized. However recent studies have shown that secreted MMPs bind to specific cell surface receptors membrane-anchored proteins or cell-associated ECM molecules and function pericellularly at focussed locations. This minireview describes examples of cell surface and pericellular partners of MMPs as well as how they alter enzyme function and cellular behaviour. doi Introduction Timely alteration of extracellular matrix ECM composition and the pericellular environment is essential in many biological processes such as embryonic development morphogenesis cell migration differentiation .