tailieunhanh - Báo cáo khoa học: Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes

We purified two hatching enzymes, namely high choriolytic enzyme (HCE; EC ) and low choriolytic enzyme (LCE; EC ), from the hatching liquid ofFundulus heteroclitus, which were named FundulusHCE (FHCE) and FundulusLCE (FLCE). FHCE swelled the inner layer of egg envelope, and FLCE completely digested the FHCE-swollen envelope. | Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes Mari Kawaguchi1 2 Shigeki Yasumasu3 Akio Shimizu4 Kaori Sano5 Ichiro Iuchi3 and Mutsumi Nishida1 1 Atmosphere and Ocean Research Institute The University of Tokyo Chiba Japan 2 Research Fellow of the Japan Society for the Promotion of Science JSPS Tokyo Japan 3 Department of Materials and Life Sciences Faculty of Science and Technology Sophia University Tokyo Japan 4 NationalResearch Institute of Fisheries Science Fisheries Research Agency Yokohama Japan 5 Graduate Program of BiologicalScience Graduate Schoolof Science and Technology Sophia University Tokyo Japan Keywords chorion egg envelope euteleostean fish Fundulus heteroclitus hatching enzyme ZP domain Correspondence M. Nishida Atmosphere and Ocean Research Institute The University of Tokyo 5-1-5 Kashiwanoha Kashiwa Chiba 277-8564 Japan Fax 81 4 7136 6211 Tel 81 4 7136 6210 E-mail mnishida@ Database The nucleotide sequence data reported in this paper are available in the EMBL GenBank DDBJ databases under the accession numbers AB533328 to AB533330 Received 6 July 2010 revised 1 October 2010 accepted 6 October 2010 doi We purified two hatching enzymes namely high choriolytic enzyme HCE EC and low choriolytic enzyme LCE EC from the hatching liquid of Fundulus heteroclitus which were named Fundulus HCE FHCE and Fundulus LCE FLCE . FHCE swelled the inner layer of egg envelope and FLCE completely digested the FHCE-swollen envelope. In addition we cloned three Fundulus cDNAs orthologous to cDNAs for the medaka precursors of egg envelope subunit proteins . choriogenins H H minor and L from the female liver. Cleavage sites of FHCE and FLCE on egg envelope subunit proteins were determined by comparing the N-termi-nal amino acid sequences of digests with the sequences deduced from the cDNAs for egg envelope subunit proteins. FHCE and FLCE cleaved .