tailieunhanh - Báo cáo y học: "Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions. | Method Open Access Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions L Ryan Baugh Joanne C Wen Andrew A Hill Donna K Slonim Eugene L Brown and Craig P Hunter Addresses Department of Molecular and Cellular Biology Harvard University Cambridge MA 02138 USA. Department of Genomics Wyeth Research Cambridge MA 02140 USA. Current address Biology Division California Institute of Technology Pasadena CA 91125. Current address Department of Computer Science Tufts University Medford MA 02155. Correspondence Craig P Hunter. E-mail hunter@ Published II April 2005 Genome Biology 2005 6 R45 doi I86 gb-2005-6-5-r45 The electronic version of this article is the complete one and can be found online at http 2005 6 5 R45 Received 2I October 2004 Revised 24 December 2004 Accepted 9 March 2005 2005 Baugh et al. licensee BioMed Central Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Phenotypic robustness is evidenced when single-gene mutations do not result in an obvious phenotype. It has been suggested that such phenotypic stability results from buffering activities of homologous genes as well as non-homologous genes acting in parallel pathways. One approach to characterizing mechanisms of phenotypic robustness is to identify genetic interactions specifically double mutants where buffering is compromised. To identify interactions among genes implicated in posterior patterning of the Caenorhabditis elegans embryo we measured synthetic lethality following RNA interference of 22 genes in 15 mutant strains. A pair of homologous T-box transcription factors tbx-8 and tbx-9 is found to interact in both C. elegans and C. briggsae indicating that their