tailieunhanh - Báo cáo y học: "Current address: Centro Nacional de Biotecnología (CNB), Campus de la Universidad Autónoma"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Current address: Centro Nacional de Biotecnología (CNB), Campus de la Universidad Autónoma . | Method Open Access A genome annotation-driven approach to cloning the human ORFeome John E Collins Charmain L Wright Carol A Edwards Matthew P Davis James A Grinham Charlotte G Cole Melanie E Goward Begona Aguado Meera Mallya Younes Mokrab Elizabeth J Huckle David M Beare and Ian Dunham Addresses The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus Hinxton Cambridge CB10 1SA UK. MRC Rosalind Franklin Centre for Genomics Research formerly MRC UK Human Genome Mapping Resource Centre Hinxton Cambridge CB10 1SB UK. Current address Department of Anatomy University of Cambridge Downing Street Cambridge CB2 3DY UK. Current address Centro Nacional de Biotecnología CNB Campus de la Universidad Autónoma de Madrid Cantoblanco 28049 Madrid Spain. Current address Cambridge Institute for Medical Research Wellcome Trust MRC Building Addenbrooke s Hospital Hills Road Cambridge CB2 2XY UK. Current address Department of Biochemistry Sanger Building University of Cambridge 80 Tennis Court Road Cambridge CB2 1GA UK. Correspondence Ian Dunham. E-mail id1@ Published 30 September 2004 Genome Biology 2004 5 R84 The electronic version of this article is the complete one and can be found online at http 2004Z5 10 R84 Received 28 May 2004 Revised 16 July 2004 Accepted 1 1 August 2004 2004 Collins et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We have developed a systematic approach to generating cDNA clones containing full-length open reading frames ORFs exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs cloned and confirmed by sequencing. We obtained clones representing 70 of genes on human chromosome 22 whereas searching .