tailieunhanh - Báo cáo y học: "A reverse genetic screen in Drosophila using a deletion-inducing mutagen"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: A reverse genetic screen in Drosophila using a deletion-inducing mutagen. | Method Open Access A reverse genetic screen in Drosophila using a deletion-inducing mutagen Knud Nairz Peder Zipperlen Charles Dearolf Konrad Basler and Ernst Hafen Addresses Zoologisches Institut Universitat Zurich Winterthurerstrasse 190 Zurich CH-8057 Switzerland. Institut fur Molekularbiologie Universitat Zurich Winterthurerstrasse 190 Zurich CH-8057 Switzerland. Massachusetts General Hospital Jackson 1402 55 Fruit St Boston MA 02114 USA. Correspondence Knud Nairz. E-mail nairz@ Published 28 September 2004 Genome Biology 2004 5 R83 The electronic version of this article is the complete one and can be found online at http 2004A5 10 R83 Received 14 July 2004 Revised 19 August 2004 Accepted 24 August 2004 2004 Nairz et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We report the use of the cross-linking drug hexamethylphosphoramide HMPA which introduces small deletions as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragmentlength polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms it should be possible to transfer this mutagenesis and detection procedure to other model systems. Background The fruitfly Drosophila melanogaster has been the prime genetic model organism for almost a century. This success story is mainly founded on countless so-called forward genetic screens designed to elucidate gene functions on the basis of their mutant phenotypes. Many of those screens reached a scale that has been termed saturating as they identify all nonredundant genes involved in a certain phenotypic .