tailieunhanh - ELECTROCHEMICAL DNA BIOSENSORS phần 4

Kể từ sinh vật biến đổi gen và các dẫn xuất của họ được sử dụng rộng rãi trong ngành công nghiệp thực phẩm, các xét nghiệm phân tích là cần thiết để cung cấp các phát hiện và của sinh vật biến đổi gen trong các mẫu thực phẩm | Disposable genosensor a new tool for the detection of NOS-terminator a genetic element present in GMOs B. Meric et al. Food Control 15 2004 621-626 Genetically modified organisms GMOs are referred to as living organisms of vegetable or animal origin in which the artificial introduction of foreign genes is able to confer novel haracteristics not found in the wild type. he EU Council regulation on traceability and labeling for GMO A threshold of is set for labeling below which labeling of iMOs is not required. Since GMOs and their derivatives are widely used in food industry the analytical assays are needed to provide the detection and the of GMOs in food matrices PCR methods based on exponential amplification of a specific GMO DNA fragment are highly sensitive but require sophisticated instruments for post-amplification steps such as gel electrophoresis and fluorescent imaging. - Alternatively an electrochemical genosensor is presented here for rapid analysis of GMO related PCR products. Nos terminator is present in nearly all GMOs due to its important in gene regulation DNA extraction and PCR was performed. From the amplification of the NOS-terminator regionfragments of 180 bp base pairs were .