tailieunhanh - Báo cáo sinh học: "Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed | Ginja et al. Genetics Selection Evolution 2010 42 18 http content 42 1 18 Ge n et i cs Selection Evolution RESEARCH Open Access Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed Catarina Ginja1 Maria CT Penedo1 Maria F Sobral2 José Matos3 Carla Borges3 Dina Neves4 Teresa Rangel-Figueiredo5 and Alfredo Cravador 6 Abstract Background Decisions to initiate conservation programmes need to account for extant variability diversity loss and cultural and economic aspects. Molecular markers were used to investigate if putative Algarvia animals could be identified for use as progenitors in a breeding programme to recover this nearly extinct breed. Methods 46 individuals phenotypically representative of Algarvia cattle were genotyped for 27 microsatellite loci and compared with 11 Portuguese autochthonous and three imported breeds. Genetic distances and factorial correspondence analyses FCA were performed to investigate the relationship among Algarvia and related breeds. Assignment tests were done to identify representative individuals of the breed. Y chromosome and mtDNA analyses were used to further characterize Algarvia animals. Gene- and allelic-based conservation analyses were used to determine breed contributions to overall genetic diversity. Results Genetic distance and FCA results confirmed the close relationship between Algarvia and southern Portuguese breeds. Assignment tests without breed information classified 17 Algarvia animals in this cluster with a high probability q . With breed information 30 cows and three bulls were identified q that could be used to reconstitute the Algarvia breed. Molecular and morphological results were concordant. These animals showed intermediate levels of genetic diversity MNA Rt Ho and He relative to other Portuguese breeds. Evidence of inbreeding was also detected Fis P . The four Algarvia bulls had Y-haplotypes .

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