tailieunhanh - Báo cáo sinh học: "Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression | Genet. Sel. Evol. 40 2008 563-579 INRA EDP Sciences 2008 DOI gse 2008015 Available online at Original article Characterization of porcine ENO3 genomic and cDNA structure polymorphism and expression Jian Wu1 Donghai Zhou2 Changyan Deng3 Xiaoxiong Wu1 Liangqi Long1 Yuanzhu Xiong3 1Department of Basic Veterinary Medicine School of Animal Sciences and School of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China 2College of Veterinary Medicine Huazhong Agricultural University Wuhan 430070 P. R. China 3Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture College of Animal Science and Technology Huazhong Agricultural university Wuhan 430070 P. R. China Received 1st December 2007 accepted 4 March 2008 Abstract - In this study a full-length cDNA of the porcine ENO3 gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately kb. Differential splicing in the 5 -untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of ENO3 is highly expressed in liver and lung while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of ENO3 is different between Yorkshire and Meishan pig breeds. Furthermore real-time polymerase chain reaction revealed that in Yorkshire pigs skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2 with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the ENO3 gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage average backfat thickness meat .