tailieunhanh - báo cáo hóa học:" Probing Specific Interaction Forces Between Human IgG and Rat Anti-Human IgG by Self-Assembled Monolayer and Atomic Force Microscopy"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Probing Specific Interaction Forces Between Human IgG and Rat Anti-Human IgG by Self-Assembled Monolayer and Atomic Force Microscopy | Nanoscale Res Lett 2010 5 1032-1038 DOI s11671-010-9598-x NANO EXPRESS Probing Specific Interaction Forces Between Human IgG and Rat Anti-Human IgG by Self-Assembled Monolayer and Atomic Force Microscopy Zhengjian Lv Jianhua Wang Guoping Chen Linhong Deng Received 21 January 2010 Accepted 29 March 2010 Published online 14 April 2010 The Author s 2010. This article is published with open access at Abstract Interaction forces between biological molecules such as antigen and antibody play important roles in many biological processes but probing these forces remains technically challenging. Here we investigated the specific interaction and unbinding forces between human IgG and rat anti-human IgG using self assembled monolayer SAM method for sample preparation and atomic force microscopy AFM for interaction force measurement. The specific interaction force between human IgG and rat anti-human IgG was found to be nN and the force required for unbinding a single pair of human IgG and rat anti-human IgG was calculated to be 144 11 pN. The results are consistent with those reported in the literatures. Therefore SAM for sample preparation combined with AFM for interaction measurement is a relatively simple sensitive and reliable technique to probe specific interactions between biological molecules such as antigen and antibody. Keywords Interaction Human IgG Rat anti-human IgG Self assembled monolayer SAM Atomic force microscopy AFM Z. Lv J. Wang G. Chen L. Deng Key Laboratory of Biorheological Science and Technology Ministry of Education Chongqing University 400044 Chongqing China Z. Lv J. Wang G. Chen L. Deng Institute of Biochemistry and Biophysics College of Bioengineering Chongqing University 400044 Chongqing China e-mail wjh@ L. Deng e-mail denglh@ 0 Springer Introduction Structure dynamics and function of biological molecules are largely determined by physical forces acting on and between the molecules. For example