tailieunhanh - Báo cáo " Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production "

We established cell suspension culture on ginseng and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. The maximum biomass yields of cell suspension culture of ginseng was obtained in medium containing 2,4-D as compared to IBA or NAA. However, ginsenoside production was much higher in IBA or NAA containing medium and 7 mg/L IBA was determined to be optimal for cell growth ( mg/L DW) and ginsenoside production ( mg/g DW) | VNU Journal of Science Natural Sciences and Technology 26 2010 191-196 Cell suspension culture Panax ginseng C. A. Meyer Role of plant growth regulators and medium composition on biomass and ginsenoside production Nguyen Trung Thanh1 . Kee Yoeup Paek2 1 Faculty of Biology Hanoi University of Science VNU 334 Nguyen Trai Hanoi Vietnam 2Department of Horticulture Chungbuk National University 361-736 Cheongju Korea Received 17 April 2009 Abstract. We established cell suspension culture on ginseng and some attempts have been made to increase ginsenoside yield of ginseng cell culture through manipulation various culture factors and process variable. The maximum biomass yields of cell suspension culture of ginseng was obtained in medium containing 2 4-D as compared to IBA or NAA. However ginsenoside production was much higher in IBA or NAA containing medium and 7 mg L IBA was determined to be optimal for cell growth mg L DW and ginsenoside production mg g DW . Addition of cytokinin BA and kinetin did not affect cell growth but ginsenoside production was increased when the medium supplemented with mg L BA or mg L kinetin. Half and full strength MS medium were found to be equally suitable for both biomass as well as ginsenoside production. At various sucrose concentration investigated 30 g L sucrose enhanced biomass yield as well as ginsenoside production and further increase of sucrose concentration upto 70 g L led to a decrease in ginsenoside accumulation and biomass production. The maximum growth and ginsenoside production was obtained for nitrogen concentration of 30 mM. Keywords Auxin cytokinin sucrose MS strength and nitrogen. 1. Introduction Ginseng Panax ginseng . Meyer which belong to the Araliaceae is one of the most variable oriental herbs. It has been used as a healing drug and health tonic in countries as Korea China and Japan since ancient times 1 . Until now ginseng has reported to contain saponins antioxidants peptides polysaccharides .

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