tailieunhanh - Báo cáo " Anti-obesity and body weight reducing effect of Fortunella japonica peel extract fractions in experimentally obese mice "

The objective of the present study was to develop an experimentally obese mice model and to investigate antiobesity and body weight reducing effect of Fortunella japonica peel extract fractions. Male mice (Mus musculus, Swiss race weighed at 14-15g) purchased from National Institute of Hygiene and Epidemiology (NIHE) were divided into 5 groups in which, the first group was fed with normal pellet diet (NPD) (Standard chow from NIHE), and other groups (2-5 groups) were fed high fat diet (HFD) containing 58% calories as fat for a period of 15 days. . | VNU Journal of Science Natural Sciences and Technology 25 2009 179-187 Anti-obesity and body weight reducing effect of Fortunella japonica peel extract fractions in experimentally obese mice Do Ngoc Lien Nguyen Thuy Quynh Nguyen Hoang Quang Nguyen Thi Thanh Ngan Faculty of Biology College of Science VNU 334 Nguyen Trai Hanoi Vietnam Received 15 April 2009 Abstract. The objective of the present study was to develop an experimentally obese mice model and to investigate antiobesity and body weight reducing effect of Fortunella japonica peel extract fractions. Male mice Mus musculus Swiss race weighed at 14-15g purchased from National Institute of Hygiene and Epidemiology NIHE were divided into 5 groups in which the first group was fed with normal pellet diet NPD Standard chow from NIHE and other groups 2-5 groups were fed high fat diet HFD containing 58 calories as fat for a period of 15 days. The HFD mice exhibited significant increase in body weight about 44 as compared to NPD group at that time p . Besides the HFD mice showed significant increase in blood total cholesterol blood triglyceride LDL and glucose concentrations to be and respectively as compared to NPD mice. In addition the effects of daily repeated oral administration for 8 days of 800 mg kg of ethylacetate chloroform and ethanol extract fractions from Fortunella japonica peels were studied. The obtained results showed that the body weights of HFD mice treated by different extract fractions were reduced by for ethylacetate extract for choloroform extract and for ethanol extract as compared to vehicle control obese mice were treated by water replaced the extracts p . Besides HFD mice treated by different extract fractions exhibited significant decrease in blood total cholesterol to for ethanol extract for chloroform extract for ethylacetate in triglycerid 13 for ethanol extract for chloroform extract for ethylacetate extract in glucose .