tailieunhanh - Báo cáo " Assessment of OPRM1 and HRH2 gene variants in Vietnamese using RFLP-PCR "

The gene encoding the mu-opioid receptor (OPRM1) has been reported to associate with a range of substance dependence and HRH2 is the gene coding histamine H2 receptor, the site of action for various compounds, . amitriptiline and mianserin, used in the treatment of psychiatric disorders. Both OPRM1and HRH2 genes contain some single nucleotide polymorphisms (SNPs), including +118A/G in OPRM1 and +398T/C in HRH2, which were reported to change in protein sequences and result in affecting the therapeutic response. . | VNU Journal of Science Natural Sciences and Technology 25 2009 228-233 Assessment of OPRM1 and HRH2 gene variants in Vietnamese using RFLP-PCR Dinh Doan Long Nguyen Thi Hong Van Nguyen Anh Luong Tran Thi Thuy Anh Faculty of Biology College of Science VNU 334 Nguyen Trai Hanoi Vietnam Received 17 December 2009 Abstract. The gene encoding the mu-opioid receptor OPRM1 has been reported to associate with a range of substance dependence and HRH2 is the gene coding histamine H2 receptor the site of action for various compounds . amitriptiline and mianserin used in the treatment of psychiatric disorders. Both OPRM1and HRH2 genes contain some single nucleotide polymorphisms SNPs including 118A G in OPRM1 and 398T C in HRH2 which were reported to change in protein sequences and result in affecting the therapeutic response. Some previous studies revealed linkage between these rare alleles and propensity towards drug dependence. This paper reports the data of an investigation on frequencies of several SNPs at OPRM1 and HRH2 loci in a Vietnamese population by using RFLP-PCR technique. For OPRM1 the IVS2 691G allele appeared with a frequency of 25 . The SNP 543G A were found at the HRH2 locus with frequency of 5 . These frequencies seemed to concur with those found in previous studíes in some other Asian populations such as Japanese Indian or Chinese but notably the frequencies of these alleles found in Vietnamese appeared to differ significantly from those found in European and African-American populations as revealed by previous investigations. Keywords mu-opioid receptor histamine H2 receptor single nucleotide polymorphism SNP PCR-RFLP. 1. Introduction Pharmacogenetic approaches provide a method for identifying mechanisms underlying interindividual response variation and in particular identifying patients who have a higher probability of benefiting from a particular medication 1 . Because medications appear to exert their therapeutic effects through Corresponding author.