tailieunhanh - báo cáo hóa học:" Reproducibility of quantitative (R)-[11C]verapamil studies"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Reproducibility of quantitative (R)-[11C]verapamil studies | van Assema et al. EJNMMI Research 2012 2 1 http content 2 1 1 9 EJNMMI Research a SpringerOpen Journal ORIGINAL RESEARCH Open Access Reproducibility of quantitative R - 11C verapamil studies 2 3 3 3 Danielle ME van Assema 1 Mark Lubberink Ronald Boellaard Robert C Schuit Albert D Windhorst Philip Scheltens1 Bart NM van Berckel3 and Adriaan A Lammertsma3 Abstract Background P-glycoprotein Pgp dysfunction may be involved in neurodegenerative diseases such as Alzheimer s disease and in drug resistant epilepsy. Positron emission tomography using the Pgp substrate tracer R - 11C verapamil enables in vivo quantification of Pgp function at the human blood-brain barrier. Knowledge of test-retest variability is important for assessing changes over time or after treatment with disease-modifying drugs. The purpose of this study was to assess reproducibility of several tracer kinetic models used for analysis of R - 11C verapamil data. Methods Dynamic R - 11C verapamil scans with arterial sampling were performed twice on the same day in 13 healthy controls. Data were reconstructed using both filtered back projection FBP and partial volume corrected ordered subset expectation maximization PVC OSEM . All data were analysed using single-tissue and two-tissue compartment models. Global and regional test-retest variability was determined for various outcome measures. Results Analysis using the Akaike information criterion showed that a constrained two-tissue compartment model provided the best fits to the data. Global test-retest variability of the volume of distribution was comparable for single-tissue 6 and constrained two-tissue 9 compartment models. Using a single-tissue compartment model covering the first 10 min of data yielded acceptable global test-retest variability 9 for the outcome measure K1. Test-retest variability of binding potential derived from the constrained two-tissue compartment model was less robust but still acceptable 22 . Test-retest .