tailieunhanh - Báo cáo hóa học: " Intracellular ZnO Nanorods Conjugated with Protoporphyrin for Local Mediated Photochemistry and Efficient Treatment of Single Cancer Cell"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Intracellular ZnO Nanorods Conjugated with Protoporphyrin for Local Mediated Photochemistry and Efficient Treatment of Single Cancer Cell | Nanoscale Res Lett 2010 5 1669-1674 DOI s11671-010-9693-z NANO EXPRESS Intracellular ZnO Nanorods Conjugated with Protoporphyrin for Local Mediated Photochemistry and Efficient Treatment of Single Cancer Cell S. Kishwar M. H. Asif O. Nur M. Willander Per-Olof Larsson Received 22 June 2010 Accepted 1 July 2010 Published online 15 July 2010 The Author s 2010. This article is published with open access at Abstract ZnO nanorods NRs with high surface area to volume ratio and biocompatibility is used as an efficient photosensitizer carrier system and at the same time providing intrinsic white light needed to achieve cancer cell necrosis. In this letter ZnO nanorods used for the treatment of breast cancer cell T47D are presented. To adjust the sample for intracellular experiments we have grown the ZnO nanorods on the tip of borosilicate glass capillaries m diameter by aqueous chemical growth technique. The grown ZnO nanorods were conjugated using protoporphyrin dimethyl ester PPDME which absorbs the light emitted by the ZnO nanorods. Mechanism of cytotoxicity appears to involve the generation of singlet oxygen inside the cell. The novel findings of cell-localized toxicity indicate a potential application of PPDME-conjugated ZnO NRs in the necrosis of breast cancer cell within few minutes. Keywords ZnO nanorods Cancer cell necrosis Photodynamic therapy Protoporphyrin dimethyl ester Introduction Zinc oxide ZnO is gaining much research attention due to many advantageous properties like direct band gap of eV and large exciton binding energy of 60 meV at S. Kishwar M. H. Asif O. Nur M. Willander Department of Science and Technology Campus Norrkoping Linkoping University 60174 Norrkoping Sweden e-mail magwi@ . Larsson Department of Pure and Applied Biochemistry Lund University Box 124 221 00 Lund Sweden room temperature and deep level defects emissions that cover the whole visible range. In fact ZnO is one of the very few .